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cytc oxidase iv  (Proteintech)


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    Proteintech cytc oxidase iv
    Animal treatment and the effects of radiation on renal function, ROS level, and expression of <t>Cytc.</t> (A) The timeline shows the time points for drug treatment and radiation. (B) SCr (a), BUN (b), and UA (c) levels in different groups of mice are shown. (C) ROS levels in different groups of mouse kidneys were evaluated by dihydroethidium staining (400×). (D) Western blot expression results and the quantification of the levels of Cytc in cytoplasm (a,c) and mitochondria (b,d) in the kidneys of each group, with normalization to GAPDH <t>and</t> <t>COX-IV</t> respectively. The data are expressed as the mean ± standard error of the mean (n=3; **, P<0.01 and ***, P<0.001 vs. the control group; # , P<0.05, ## , P<0.01 and ### , P<0.001 vs. the DMSO group). ROS, reactive oxygen species; Cytc, cytochrome C; AS-IV, astragaloside IV; CsA, cyclosporin A; SCr, creatinine; BUN, blood urea nitrogen; UA, uric acid; DMSO, dimethyl sulfoxide; IR, irradiation; c-Cyt c, Cytc in the cytosol; COX-IV, Cytc oxidase IV; mito-Cyt c, Cytc in the mitochondria.
    Cytc Oxidase Iv, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cytc oxidase iv/product/Proteintech
    Average 95 stars, based on 112 article reviews
    cytc oxidase iv - by Bioz Stars, 2026-02
    95/100 stars

    Images

    1) Product Images from "Suppression of NLRP3 inflammasome activation by astragaloside IV via promotion of mitophagy to ameliorate radiation-induced renal injury in mice"

    Article Title: Suppression of NLRP3 inflammasome activation by astragaloside IV via promotion of mitophagy to ameliorate radiation-induced renal injury in mice

    Journal: Translational Andrology and Urology

    doi: 10.21037/tau-23-323

    Animal treatment and the effects of radiation on renal function, ROS level, and expression of Cytc. (A) The timeline shows the time points for drug treatment and radiation. (B) SCr (a), BUN (b), and UA (c) levels in different groups of mice are shown. (C) ROS levels in different groups of mouse kidneys were evaluated by dihydroethidium staining (400×). (D) Western blot expression results and the quantification of the levels of Cytc in cytoplasm (a,c) and mitochondria (b,d) in the kidneys of each group, with normalization to GAPDH and COX-IV respectively. The data are expressed as the mean ± standard error of the mean (n=3; **, P<0.01 and ***, P<0.001 vs. the control group; # , P<0.05, ## , P<0.01 and ### , P<0.001 vs. the DMSO group). ROS, reactive oxygen species; Cytc, cytochrome C; AS-IV, astragaloside IV; CsA, cyclosporin A; SCr, creatinine; BUN, blood urea nitrogen; UA, uric acid; DMSO, dimethyl sulfoxide; IR, irradiation; c-Cyt c, Cytc in the cytosol; COX-IV, Cytc oxidase IV; mito-Cyt c, Cytc in the mitochondria.
    Figure Legend Snippet: Animal treatment and the effects of radiation on renal function, ROS level, and expression of Cytc. (A) The timeline shows the time points for drug treatment and radiation. (B) SCr (a), BUN (b), and UA (c) levels in different groups of mice are shown. (C) ROS levels in different groups of mouse kidneys were evaluated by dihydroethidium staining (400×). (D) Western blot expression results and the quantification of the levels of Cytc in cytoplasm (a,c) and mitochondria (b,d) in the kidneys of each group, with normalization to GAPDH and COX-IV respectively. The data are expressed as the mean ± standard error of the mean (n=3; **, P<0.01 and ***, P<0.001 vs. the control group; # , P<0.05, ## , P<0.01 and ### , P<0.001 vs. the DMSO group). ROS, reactive oxygen species; Cytc, cytochrome C; AS-IV, astragaloside IV; CsA, cyclosporin A; SCr, creatinine; BUN, blood urea nitrogen; UA, uric acid; DMSO, dimethyl sulfoxide; IR, irradiation; c-Cyt c, Cytc in the cytosol; COX-IV, Cytc oxidase IV; mito-Cyt c, Cytc in the mitochondria.

    Techniques Used: Expressing, Staining, Western Blot, Control, Irradiation



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    Animal treatment and the effects of radiation on renal function, ROS level, and expression of <t>Cytc.</t> (A) The timeline shows the time points for drug treatment and radiation. (B) SCr (a), BUN (b), and UA (c) levels in different groups of mice are shown. (C) ROS levels in different groups of mouse kidneys were evaluated by dihydroethidium staining (400×). (D) Western blot expression results and the quantification of the levels of Cytc in cytoplasm (a,c) and mitochondria (b,d) in the kidneys of each group, with normalization to GAPDH <t>and</t> <t>COX-IV</t> respectively. The data are expressed as the mean ± standard error of the mean (n=3; **, P<0.01 and ***, P<0.001 vs. the control group; # , P<0.05, ## , P<0.01 and ### , P<0.001 vs. the DMSO group). ROS, reactive oxygen species; Cytc, cytochrome C; AS-IV, astragaloside IV; CsA, cyclosporin A; SCr, creatinine; BUN, blood urea nitrogen; UA, uric acid; DMSO, dimethyl sulfoxide; IR, irradiation; c-Cyt c, Cytc in the cytosol; COX-IV, Cytc oxidase IV; mito-Cyt c, Cytc in the mitochondria.
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    Animal treatment and the effects of radiation on renal function, ROS level, and expression of <t>Cytc.</t> (A) The timeline shows the time points for drug treatment and radiation. (B) SCr (a), BUN (b), and UA (c) levels in different groups of mice are shown. (C) ROS levels in different groups of mouse kidneys were evaluated by dihydroethidium staining (400×). (D) Western blot expression results and the quantification of the levels of Cytc in cytoplasm (a,c) and mitochondria (b,d) in the kidneys of each group, with normalization to GAPDH <t>and</t> <t>COX-IV</t> respectively. The data are expressed as the mean ± standard error of the mean (n=3; **, P<0.01 and ***, P<0.001 vs. the control group; # , P<0.05, ## , P<0.01 and ### , P<0.001 vs. the DMSO group). ROS, reactive oxygen species; Cytc, cytochrome C; AS-IV, astragaloside IV; CsA, cyclosporin A; SCr, creatinine; BUN, blood urea nitrogen; UA, uric acid; DMSO, dimethyl sulfoxide; IR, irradiation; c-Cyt c, Cytc in the cytosol; COX-IV, Cytc oxidase IV; mito-Cyt c, Cytc in the mitochondria.
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    Image Search Results


    Animal treatment and the effects of radiation on renal function, ROS level, and expression of Cytc. (A) The timeline shows the time points for drug treatment and radiation. (B) SCr (a), BUN (b), and UA (c) levels in different groups of mice are shown. (C) ROS levels in different groups of mouse kidneys were evaluated by dihydroethidium staining (400×). (D) Western blot expression results and the quantification of the levels of Cytc in cytoplasm (a,c) and mitochondria (b,d) in the kidneys of each group, with normalization to GAPDH and COX-IV respectively. The data are expressed as the mean ± standard error of the mean (n=3; **, P<0.01 and ***, P<0.001 vs. the control group; # , P<0.05, ## , P<0.01 and ### , P<0.001 vs. the DMSO group). ROS, reactive oxygen species; Cytc, cytochrome C; AS-IV, astragaloside IV; CsA, cyclosporin A; SCr, creatinine; BUN, blood urea nitrogen; UA, uric acid; DMSO, dimethyl sulfoxide; IR, irradiation; c-Cyt c, Cytc in the cytosol; COX-IV, Cytc oxidase IV; mito-Cyt c, Cytc in the mitochondria.

    Journal: Translational Andrology and Urology

    Article Title: Suppression of NLRP3 inflammasome activation by astragaloside IV via promotion of mitophagy to ameliorate radiation-induced renal injury in mice

    doi: 10.21037/tau-23-323

    Figure Lengend Snippet: Animal treatment and the effects of radiation on renal function, ROS level, and expression of Cytc. (A) The timeline shows the time points for drug treatment and radiation. (B) SCr (a), BUN (b), and UA (c) levels in different groups of mice are shown. (C) ROS levels in different groups of mouse kidneys were evaluated by dihydroethidium staining (400×). (D) Western blot expression results and the quantification of the levels of Cytc in cytoplasm (a,c) and mitochondria (b,d) in the kidneys of each group, with normalization to GAPDH and COX-IV respectively. The data are expressed as the mean ± standard error of the mean (n=3; **, P<0.01 and ***, P<0.001 vs. the control group; # , P<0.05, ## , P<0.01 and ### , P<0.001 vs. the DMSO group). ROS, reactive oxygen species; Cytc, cytochrome C; AS-IV, astragaloside IV; CsA, cyclosporin A; SCr, creatinine; BUN, blood urea nitrogen; UA, uric acid; DMSO, dimethyl sulfoxide; IR, irradiation; c-Cyt c, Cytc in the cytosol; COX-IV, Cytc oxidase IV; mito-Cyt c, Cytc in the mitochondria.

    Article Snippet: This was followed by blocking in 5% skimmed milk in tris-buffered saline with Tween20 (TBST) at RT for 1 h and overnight incubation with primary antibodies against c-Cytc (bs-0013R; Bioss Antibodies), NLRP3, cleaved caspase-1 (bs-10442R; Bioss Antibodies), IL-1β (bs-0812R; Bioss), Bax (A00183; Boster Bio), B-cell lymphoma-2 (Bcl-2; BA0412; Boster Bio), prostacyclin 62 (P62; bs-2951R; Bioss Antibodies), parkin (bs-23687R; Bioss Antibodies), LC3, PINK1 (23274-1-AP; Proteintech), cleaved caspase-3, cleaved caspase-9 (20750S; Cell Signaling Technology, Danvers, MA, USA), Cytc oxidase IV (COX-IV; 11242-1-AP; Proteintech), β-actin (bs-0061R; Bioss Antibodies), and GAPDH (K200057M, Solarbio) at 4 °C, with subsequent incubation in corresponding HRP-conjugated secondary antibodies at RT for 2 hours.

    Techniques: Expressing, Staining, Western Blot, Control, Irradiation